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CellSignalingTechnology(CST)是一家專業(yè)的細(xì)胞通路研究產(chǎn)品和服務(wù)提供商，提供抗體、試劑、蛋白質(zhì)組學(xué)、試劑盒和耗材，以及免疫學(xué)、神經(jīng)退行性疾病、表觀遺傳學(xué)等解決方案。
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CST Anti-rabbit IgG, HRP-linked Antibody產(chǎn)品參數(shù)
| 供貨周期 | 一周 | 規(guī)格 | 1ml |
| 貨號 | 7074S |
CST Anti-rabbit IgG, HRP-linked Antibody詳細(xì)信息
Product Usage Information
Recommended Antibody Dilutions:
1:1000–1:3000
20X LumiGLO® Reagent and 20X Peroxide #7003 1:1K–1:3K
SignalFireTM ECL Reagent #6883 1:1K–1:3K
SignalFireTM Plus ECL Reagent #12630 1:5K-1:15K
SignalFireTM Elite ECL Reagent #12757 1:10K-1:20K
Storage
Supplied in 10mM sodium HEPES (pH 7.5), 150 mM NaCl, 2 mg/ml bovine serum albumin (BSA) and 50% glycerol. Store at –20°C. Do not aliquot the antibodies. This product is stable for 60 months when stored at -20C.
Product Description
Designed for use with rabbit polyclonal and monoclonal antibodies, this affinity purified goat anti-rabbit IgG (heavy and light chain) antibody is conjugated to horseradish peroxidase(HRP) for chemiluminescent detection. This product is thoroughly validated with CST primary antibodies and will work optimally with the CST western immunoblotting protocol, ensuring accurate and reproducible results.
Background
Chemiluminescence systems have emerged as the best all-around method for western blot detection. They eliminate the hazards associated with radioactive materials and toxic chromogenic substrates. The speed and sensitivity of these methods are unequalled by traditional alternatives, and because results are generated on film, it is possible to record and store data permanently. Blots detected with chemiluminescent methods are easily stripped for subsequent reprobing with additional antibodies. HRP-conjugated secondary antibodies are utilized in conjunction with specific chemiluminescent substrates to generate the light signal. HRP conjugates have a very high turnover rate, yielding good sensitivity with short reaction times.
Species Reactivity
Species reactivity is determined by testing in at least one approved application (e.g., western blot).
Applications Key
WB: Western Blotting
Cross-Reactivity Key
H: human M: mouse R: rat Hm: hamster Mk: monkey Vir: virus Mi: mink C: chicken Dm: D. melanogaster X: Xenopus Z: zebrafish B: bovine Dg: dog Pg: pig Sc: S. cerevisiae Ce: C. elegans Hr: horse GP: Guinea Pig Rab: rabbit All: all species expected
僅限科研使用
For Research Use Only. Not for Use in Diagnostic Procedures.
Applications:
WB
Source/Isotype:
Goat
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