近日，深圳市護家科技有限公司在《Frontiers in Pharmacology》發(fā)表了文獻《Synergistic effects of retinol and retinyl palmitate in alleviating UVB-induced DNA damage and promoting the homologousrecombination repair in keratinocytes》，文獻中的實驗采用了上海儀橙科技有限公司的的UCL-3500M紫外交聯(lián)儀，UCL-3500M安裝了6根15w UVB紫外線燈管，內(nèi)置了紫外線傳感器，能夠自動測量輻照能量，精準(zhǔn)控制實驗過程輻照的紫外線能量，UCL-3500M紫外交聯(lián)儀是科研院所、化妝品以及化妝品原材料企業(yè)進行光毒性實驗的非常好用的實驗設(shè)備。

深圳市護家科技有限公司是深圳的一家高科技公司，正式成立于2014年7月，護家科技旗下?lián)碛锌蒲屑壒πёo膚品牌HBN，HBN堅持以“擁有看得見效果”的創(chuàng)新理念，專研打造功效的護膚品。致力于讓用戶把每一分錢都花在有效成分上 。深圳護家自建具有CNAS認(rèn)證的科研實驗室，包括細(xì)胞、理化、 植物原料、配方機制、臨床功效等科研實驗室，堅持自主創(chuàng)新研發(fā)。核心研發(fā)團隊來自國內(nèi)外科研院所工科博士、碩士等科研專家團隊。

文獻摘要：

Background: Ultraviolet B (UVB) rays are a type of ultraviolet radiation emitted by the sun, primarily responsible for skin photodamage. These rays mainly affect the epidermis, leading to direct damage to DNA and contributing to skin cancer development. Retinol and its derivatives are effective in combating skin aging and photodamage, but they often cause skin intolerance, limiting their use despite their potent effects. Therefore, investigating optimal compositions of retinoids is essential to enhance their efficacy against photodamage.

Method: In this study, we investigated the synergistic effects of retinol (ROL) and retinyl palmitate (RPalm) in alleviating UVB-induced DNA damage in human keratinocytes (HaCaT) and reconstructed human epidermis. The ROL+RPalm combination was applied after UVB exposure. We utilized bulk mRNA sequencing, comet assays, Western blotting, immunofluorescence, and flow cytometry to evaluate the level of DNA damage and repair.

Result: The application of the ROL+RPalm combination significantly reduced inflammation and apoptosis while promoting collagen synthesis compared to individual treatments with ROL or RPalm. Our findings indicated that the ROL+RPalm synergy primarily mediates DNA damage repair. Additionally, we elucidated that the molecular mechanism involves the activation of RARβ, which triggers the ATM-CHK2-p53 signaling pathway and increases the expression of homologous recombination (HR)-associated repair genes. Conclusion: This combination of ROL and RPalm presents a potential therapeutic strategy for UVB-induced photodamage and emphasizes the synergistic effects in alleviating UVB-induced DNA damage。