Development and validation of an efficient and sensitive LC-MS/MS assay for simultaneous determination of the high frequency used antibody drug conjugate payload MMAE and anti-vascular tyrosine kinase inhibitor lenvatinib in human plasma
Advanced Mass Spectrometry Strategies for Deciphering Structural and Functional Aspects of Biomolecular Complexes
doi.org/10.3929/ethz-c-000789649
藥物類(lèi)型：DNA編碼化合物庫(kù) (帶有單鏈/雙鏈 DNA 標(biāo)簽的配體)
文獻(xiàn)概述：該頂尖質(zhì)譜研究深入探討了 DNA 標(biāo)簽對(duì)小分子配體結(jié)合親和力的影響。在分析帶有單鏈或雙鏈 DNA 的復(fù)雜極性復(fù)合物時(shí)，常規(guī) C18 柱難以保留。借助極性改性色譜柱（如 Luna Omega Polar C18），研究人員在質(zhì)譜前端實(shí)現(xiàn)了強(qiáng)極性 DNA-配體復(fù)合物的高效保留與分離，為后續(xù)靶點(diǎn)親和力質(zhì)譜測(cè)定奠定了基礎(chǔ)。
如果您也做類(lèi)似研究，我們推薦以下色譜柱產(chǎn)品：
Phenomenex色譜柱型號(hào)及規(guī)格：
Luna® Omega 1.6 µm Polar C18 (50 x 2.1 mm, 100?) / Kinetex® 核殼柱
具體應(yīng)用場(chǎng)景：
針對(duì)帶有強(qiáng)極性大分子 DNA 標(biāo)簽的 DEL 庫(kù)分子，進(jìn)行高分辨率的 LC-MS/MS 結(jié)構(gòu)表征及結(jié)合親和力分析。
【從 DEL 篩選到高潛力候選藥物的轉(zhuǎn)化與合成】
文獻(xiàn)標(biāo)題:
1. From DNA-Encoded Library Screening to AM-9747: An MTA-Cooperative PRMT5 Inhibitor with Potent Oral In Vivo Efficacy (J. Med. Chem., 2025)
doi.org/10.1021/acs.jmedchem.4c03101
2. DNA-encoded library screening uncovers potent DNMT2 inhibitors targeting a cryptic allosteric binding site (iScience, 2025)
新型共價(jià)分子膠 (VVD-065) 體內(nèi)抗腫瘤試驗(yàn)（In vivo efficacy）中的血漿藥代動(dòng)力學(xué) LC-MS 監(jiān)控，及其原料分子的制備分離。
上下滑動(dòng)查看更多參考文獻(xiàn)
1.Yeast Display Technology Enables Rapid Discovery of Low-Nanomolar Macrocyclic Peptide Inhibitors of Human Angiotensin-Converting Enzyme 2.
2.Differential Protein Precipitation-Based GalNAc-siRNA Sample Preparation with LC/MS Method Development Workflow in Plasma.
3.Development and validation of an efficient and sensitive LC-MS/MS assay for simultaneous determination of the high frequency used antibody drug conjugate payload MMAE and anti-vascular tyrosine kinase inhibitor lenvatinib in human plasma.
4.Synthesis of integrin-targeting pro-drugs for the selective release of anti-tumor agents.
5.Advanced Mass Spectrometry Strategies for Deciphering Structural and Functional Aspects of Biomolecular Complexes.
6.From DNA-Encoded Library Screening to AM-9747: An MTA-Cooperative PRMT5 Inhibitor with Potent Oral In Vivo Efficacy (J. Med. Chem., 2025).
7.DNA-encoded library screening uncovers potent DNMT2 inhibitors targeting a cryptic allosteric binding site (iScience, 2025).
8.An engineered cereblon optimized for high-throughput screening and molecular glue discovery.
9.A covalent allosteric molecular glue suppresses NRF2-dependent cancer growth.
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